Northern using fluorescein-probes
p.r.ashby at MAPS.dundee.ac.uk
Tue Feb 12 15:15:12 EST 2002
In article <KHda8.7653$tv6.196083 at twister1.libero.it>,
"Stefi~" <gifalone at tin.it> wrote:
> I'm trying to do a Northern using a single 5'-fluorescein-DNA probe. A
> couldn't find any references about using this kind of labelled-probe in
> Northern, in fact everybody uses radiolabelled (32P) probes.
> Does anyone know about Northern blotting made using fluorescein-DNA probes?
> I'd like some references.
Look on Roche's website or their catalogue. Basically you need the Alk
Phos labelled Anti FITC antibody and their chemiluminiscence kit to do
Wellcome Trust Biocentre
University of Dundee, Scotland
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