Tgo polymerase

Susanne Rohrer rohrersusanne.spam at hotmail.com
Thu Feb 14 06:34:23 EST 2002



Michael Witty wrote:

> On Tue, 12 Feb 2002, Susanne Rohrer wrote:
>
> > Hi,
> > has anyone tried Tgo polymerase from Roche? I have, and am not happy
> > with it (I admit I only tried my most recent primer pair that worked
> > excellently with Pwo)
> > They just ran out of Pwo because it was popular, what a blunder.
> > I am wondering what other people think...
>
> I think that sometimes when you change an enzyme you need to change
> reaction conditions too.  Mike.

That's exactly what they say you don't have to - in addition, i would
hate not to be able to trust my female intuition about melting
temperatures anymore. we have also been using Pwo in a routine
application and will have to do the whole evaluation again.





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