Stable transfection - how?

Leman Leman at Leman.org
Fri Feb 15 11:15:52 EST 2002


Emir,
Other selection compounds we use are zeocin and puromycin. Zeocin is
very similar to G418 in terms of doses used and time of selection
(500-1000 µg/mL for 2 weeks in my cases). Puromycin - I never used it,
so I don't know doses (people next door use 1 µg/mL), and it kills
cells much faster - within 3-5 days.
Good luck.
Still remembering U of C fondly,

Leman

P.S. In case your reader is set up differently (lets say, to KOI-8),
by "µ" I meant "micro"




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