isolate the nucleus intact

kent compsci2003 at
Fri Feb 15 12:33:55 EST 2002

hello, i am interested in isolating intact nuclei from yeast cultures
to later obtain the nuclear extract.if anyone has a protocol to
isolate yeast nulei intact, please email it to me at
compsci2003 at
I will also be doing the same with various cell types, any protocols
that will allow me to isolate intact nuclei would be welcome. thanks
for your time.

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