missing nucleotide close to the primer

Roland Hübner roland.hubner at ua.ac.be
Mon Feb 18 09:48:54 EST 2002

> Is it possible to miss a nucleotide in a stretch of about 60 nucleotides 
> close to the primer that was used for the sequencing reaction ?? I got a
> nucleotide deleted (missing) from two different DNA sequences...

 Hmmmm, are these seqs from topoisomerase-cloned products ?


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