SDS PAGE of LB medium

Michael Witty mw132 at mole.bio.cam.ac.uk
Mon Feb 18 13:27:34 EST 2002


Dear All,
        I am going to try some SDS-PAGE of the medium my E. coli are
growing in tomorrow - to see if my desired protein is secreted into the
medium.  BUT how much should I load?  I am going to TCA ppt some to avoid
salt, but should I expect a large amount of spurious protein or hardly any
at all?  Plan A is to load ppt from 25microlitres per gel lane.
Regards, Mike.




More information about the Methods mailing list