SDS PAGE of LB medium

Michael Witty mw132 at
Mon Feb 18 13:27:34 EST 2002

Dear All,
        I am going to try some SDS-PAGE of the medium my E. coli are
growing in tomorrow - to see if my desired protein is secreted into the
medium.  BUT how much should I load?  I am going to TCA ppt some to avoid
salt, but should I expect a large amount of spurious protein or hardly any
at all?  Plan A is to load ppt from 25microlitres per gel lane.
Regards, Mike.

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