RNAse inhibitors and DNAse1 treatment

aes ali at image.dk
Wed Feb 20 06:53:28 EST 2002


Hi.
I am forced to DNAse1 treat my RNA samples for a quite a long time (at 37
degrees C) to remove contaminating DNA. I have thought about adding RNAsin
or RNAseOut inhibitor (or other) to the mix when I do the DNAse treatment.
My question is if these commercial RNAse inhibitors also inhibits the DNAse
enzyme? And do these inhibitors function at 37 degrees?
Thank you
AES





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