Expression of membrane proptein

rajneeli rajneeli at lebs.cnrs-gif.fr
Fri Feb 22 04:57:40 EST 2002


I have some problem in expression of membrane protein. I am using
pKK-223 vector and E. Coli JM105 for the expression. Even though I
have expression, it is very little.

How to increase yield of my protein,
What are the specific problems particularly for the expression of
memebranous proteins.

Thanking you in advance

Rajasekhar




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