Expression of membrane proptein
legatek at mcmail.cis.mcmaster.ca
Sat Feb 23 14:01:13 EST 2002
On 22 Feb 2002, rajneeli wrote:
> I have some problem in expression of membrane protein. I am using
> pKK-223 vector and E. Coli JM105 for the expression. Even though I
> have expression, it is very little.
E. coli have only 1 membrane, so if you're expressing a membrane-bound
version of your protein it should come as no surprise that the expression
is poor. I think you have 3 options:
1. Accept the low expression level and work with it,
2. increase the expression level through experimenting with different
promoter constructs and deal with the inclusion body mess
3. generate a truncated, soluble version of your protein or fuse it to
something that renders it soluble.
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legatek at mcmaster.ca Kyle Legate legatek at hotmail.com
Tower of Tongues:Thursday PM:10:30-11:30 EDT:http://cfmu.mcmaster.ca
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