PCR clonning

SerAln SerREMOVETHISAln at netscape.net
Mon Feb 25 15:27:52 EST 2002


do you perform blue/white selection?
are you sure the insert is in there?
are you using a T/A system and Taq pol, blunt ends ligation or PCR restriction 
into a linearized plasmid?
have you add the correct restriction sequences to the primers?

Sergio

erika wrote:

>  I have not been able to get the correct clone from the transformation. I
> extracted DNA plasmid but when I digest I don´t get any desired fragment. Do
> I have to linearize befores the restriction assay?
> 
> 
> <http://www.biowww.net/forum/read.php?f=1&i=5297&t=5297>
> 




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