retrovirus packaging qusation
wolfsc at ibms.sinica.edu.tw
Tue Feb 26 00:41:06 EST 2002
I have some difficulties understanding the difference between helper systems
and helper-free packaging systems:
In both cases, to my knowledge one transfects a construct containing the gene
of interest between the two LTRs into a packaging cell line carrying the other
genes (gag, pol, env) necessary to generate and bud the retrovirus. What's the
Thanks for all input.
Dr. Wolfgang Schechinger
Institute of Biomedical Sciences
Academia Sinica, Taipei, Taiwan R.o.C.
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