retrovirus packaging qusation

Wolfgang Schechinger wolfsc at ibms.sinica.edu.tw
Tue Feb 26 00:41:06 EST 2002


Dear all, 

I have some difficulties understanding the difference between helper systems 
and helper-free packaging systems: 

In both cases, to my knowledge one transfects a construct containing the gene 
of interest between the two LTRs into a packaging cell line carrying the other 
genes (gag, pol, env) necessary to generate and bud the retrovirus. What's the 
difference?

Thanks for all input.

Regards,
Wo

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Dr. Wolfgang Schechinger
Institute of Biomedical Sciences
Academia Sinica, Taipei, Taiwan R.o.C.

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