retrovirus packaging qusation

Wolfgang Schechinger wolfsc at
Tue Feb 26 00:41:06 EST 2002

Dear all, 

I have some difficulties understanding the difference between helper systems 
and helper-free packaging systems: 

In both cases, to my knowledge one transfects a construct containing the gene 
of interest between the two LTRs into a packaging cell line carrying the other 
genes (gag, pol, env) necessary to generate and bud the retrovirus. What's the 

Thanks for all input.


Dr. Wolfgang Schechinger
Institute of Biomedical Sciences
Academia Sinica, Taipei, Taiwan R.o.C.


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