Inducible copy number plasmid

Duncan Clark junk at 127.0.0.1
Wed Jan 2 04:34:41 EST 2002


In message <3C2CE484.316A0309 at genome.wi.mit.edu>, Peter Ianakiev 
<ianakiev at genome.wi.mit.edu> writes on the Fri, 28 Dec 2001
>We need a stable (low copy number) plasmid, that could be induced to
>high copy number before sequencing. Any ideas?

Do a search for runaway vectors, based on say pKN402 or similar. copy 
numbers of 5-10 at 28C and several thousand upon induction and growth at 
37-42C. The name Uhlin springs to mind.

Duncan
-- 
The problem with being on the cutting edge is that you occasionally get
sliced from time to time....

Duncan Clark
GeneSys Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382




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