Isolation of Cytosol. protein

Mad Scientist isl48143 at image.dk
Wed Jan 2 10:28:06 EST 2002


Cobalt resin is an option. I never used it myself though, but I've heard
that i should solve problems as yours now and then

cheers


"Tariq Hamid" <asterhamid at yahoo.com> wrote in message
news:394453f3.0112311040.2c27721f at posting.google.com...
> Hi there
> I am also trying to purify a his tagged protein from E.coli. i do get
> the protein from the Ni-NTA resin but it isa contaminbated with other
> proteins also.I have heard about using glyvero. Can you tell me more
> about that.
> Tariq
>  jhaines at uoguelph.ca (Jeffery Haines) wrote in message
news:<9ugplf$j3t$1 at testinfo.cs.uoguelph.ca>...
> > I am using Ni-NTA resin to purify a cytosolic protein. I was getting
> > lower  order bands on the SDS-PAGE gels. I tried to purify the protein
in
> > the presence of 40% glycerol, and I got rid of the lower order
> > bands but now I am getting some higher order bands.
> > Has anyone experienced a similar problem with their cytosolic protein
when
> > they try to purify in the presence of glycerol?   Any suggestions would
> > be greatly appreciated.
> >
> > Jaspreet Kaur (jkaur at uoguelph.ca)
> > University of Guelph
> >
> > //Jeff Haines (jhaines at uoguelph.ca)





More information about the Methods mailing list