Short PCR product

aes aes at
Sat Jan 5 05:35:50 EST 2002

I am going to run PCR with 35 bp long primers, with an amplicon in between
of 15 bp, so that the total lenght of the PCR product is 85 bp (for
quantitation by real time pcr and sybr green1). My question is, what is the
shortest posible amplicon? And is 15 bp to short?
Thank you and happy new year

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