Short PCR product

Duncan Clark junk at hgmp.mrc.ac.uk
Mon Jan 7 06:18:18 EST 2002


In message <9FAZ7.13912$Zm5.1213129 at news000.worldonline.dk>, aes 
<aes at donnotreply.com> writes on the Sat, 5 Jan 2002
>I am going to run PCR with 35 bp long primers, with an amplicon in between
>of 15 bp, so that the total lenght of the PCR product is 85 bp (for
>quantitation by real time pcr and sybr green1). My question is, what is the
>shortest posible amplicon? And is 15 bp to short?

Should work fine. The primers are a little long but that shouldn't 
matter for Sybr.

We've run I think 75bp in total and that included a Taqman probe in the 
middle!

Duncan

-- 
The problem with being on the cutting edge is that you occasionally get
sliced from time to time....

Duncan Clark
GeneSys Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382




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