Sepharose spin columns

marek mks at plantpath.wisc.edu
Mon Jan 7 09:53:32 EST 2002


I'd like to develop a protocol to purify DNA extracted from soil using
Sepharose CL-2B resin in 96-well spin columns. I've found references
describing Sepharose 4B spin columns and a kit using Sepharose CL-6B.
Does anyone have experience with this type of work? I'd like to get a
better sense of spin times and speeds using CL-2B for spin columns.

-marek sliwinski
mks at plantpath.wisc.edu
UW-Madison





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