about in vitro transcription

wen xzwzb at ust.hk
Mon Jan 7 22:54:56 EST 2002

Dear all:

    I wonder that: before doing in vitro transcription, when the target gene
is cloned into the right vector, should I add cap or signal peptide for
later expressing the mRNA product(membrane protein) in xenopus oocyte?
     Thanks a lot! 


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