tutter at mac.com
Mon Jan 7 22:53:59 EST 2002
hi all, a few questions regarding rNTP analogs:
1) are there any rNTP analogs other than 3'-deoxy analogs that can function
as chain-terminating NTPS in RNA synthesis? (i.e., O-methyl rNTPs, etc...)
2) is O-methyl rGTP hydrolyzable? i.e., can it substitute for GTP in
3) can GTP-gamma-S incorporate into RNA, or is it chain-terminating?
basically, i am looking for two separate things: chain-terminating rNTPs
that will *not* interfere with NTP hydrolysis, and the converse:
non-hydrolyzable NTPs that are also non-chain-terminating.
anton tutter, ph.d.
harvard medical school
dept. of biochemistry and molecular pharmacology
walter lab, c2-322
240 longwood ave.
boston, ma 02115
email: anton_tutter at hms.harvard.edu
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