slide storage ?

Thierry Giffon tgiffon at stanford.edu
Wed Jan 9 15:12:58 EST 2002


Hello everybody and Happy new year !!!!

I'd like to know if anybody would know a technique to keep slides for
Immuno-histochemistry. On these slides I have T cells or thymocytes that
I "attached" with Poly-L-Lysine. I tried fixing with PFA 2-4%, washing
and dehydration but the cells didn't look good the next day. I would
hate having to do my staining on the day I have to prepare and purify T
cell subsets !!!!
If anybody knows a trick, please make my year easier !!!

Thanks a lot in advance for your help
Thierry





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