most constant gene during development

R. John Lye rjl6n at virginia.edu
Thu Jan 10 15:03:51 EST 2002


Stefi~ wrote:

> "Nick Theodorakis" <nicholas_theodorakis at urmc.rochester.edu> ha scritto nel
> messaggio news:kr5%7.10935$cD4.23885 at www.newsranger.com...
> > I would be surprised if you can find one. There can be huge changes in
> gene
> > expression patterns and levels during differentiation and development.
> >
> > Maybe you should tell us what problem you are trying to solve.
>
> I'm trying to reveal if there is an accumulation of a specific mRNA during
> Bufo's development. To avoid working with mRNA and Northern, I want to
> convert my specific mRNAs in ssDNA using fluorescein-dUTP and Reverse
> Trascriptase. But, my signals, to be published, need to be normalized versus
> a gene which mantains constant levels during development.

Actually, to have meaningful numbers, you'd have to do that with the
Northern, too.

John






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