does anyone use Northern here?

Stefi~ gifalone at tin.it
Fri Jan 11 15:35:13 EST 2002


"Tim Spahlinger" <txs at po.cwru.edu> ha scritto nel messaggio
news:3C3F3FCF.536A1529 at po.cwru.edu...
> Stefi,
>
> Have you considered doing real-time PCR, ala TaqMan or Light
> Cycler, to relatively quantify your 2 messages?  If you know
> the sequences that you're investigating you might be able to
> design 2 FRET probes whose fluorescence would indicate the
> relative amounts of message.  The Applied Biosystems web
> site (for TaqMan chemistry; www.appliedbiosystems.com) and
> the web site for a company called Molecular Probes
> (www.molecularprobes.com; they have a handbook there that
> you could look at) could help you determine if this is right
> for you.  I'm not affiliated with either company but I am
> familiar with their technology and reagents.

Well, actually in our lab there's no avaivable any real time RT-PCR
thermalcycler. That's our problem. Thanx anyway.
Stefano
gifalone at tin.it
http://web.tiscalinet.it/gifalone





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