Northern Blot troubles
jakku71 at yahoo.com
Fri Jan 11 15:56:25 EST 2002
Try washing the membranes better, or you should try reducing the
concentration of the probes used. Try halving the concentration. The probe
may be overloaded.
> From: "Theo Roemen" <theo.roemen at fys.unimaas.nl>
> Organization: Universiteit Maastricht
> Date: Fri, 11 Jan 2002 09:23:29 +0100
> To: methods at hgmp.mrc.ac.uk
> Subject: Northern Blot troubles
> We separate RNA by Northern blotting and detection with labeled probes.
> Since a few month we get many black spots on the membrane after using 32P-
> labeled probes and after auto-radiography. Does anyone know what could be
> the cause of this?
Do You Yahoo!?
Get your free @yahoo.com address at http://mail.yahoo.com
More information about the Methods