Northern Blot troubles

Jayakumar R jakku71 at
Fri Jan 11 15:56:25 EST 2002

Try washing the membranes better, or you should try reducing the
concentration of the probes used.  Try halving the concentration.  The probe
may be overloaded.

> From: "Theo Roemen" <theo.roemen at>
> Organization: Universiteit Maastricht
> Date: Fri, 11 Jan 2002 09:23:29 +0100
> To: methods at
> Subject: Northern Blot troubles
> We separate RNA by Northern blotting and detection with labeled probes.
> Since a few month we get many black spots on the membrane after using 32P-
> labeled probes and after auto-radiography. Does anyone know what could be
> the cause of this?

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