Whici Anti-RNAse?

Dr. James J. Campanella campanellj at mail.montclair.edu
Mon Jan 14 08:11:02 EST 2002


Hi,

The best, most efficient anti-RNase agent that we have
found is RNA Secure from Ambion. The best thing about it 
is that it can be left in most enzymatic reactions. It does 
not interfere at all with reactions like RT-PCR. Glassware and
plastic-ware can be treated with a 1/12th solution. The 
anti-RNase activity is quite stable and can be reactivated an 
indeterminate number of times when the solution is heated to
above 45C.

regards,
Jim


At 02:44 AM 1/14/2002 +0000, Wolfgang Schechinger wrote:
>Dear JP,
>
>looks like sodium hydroxide +- bleach + rinse with DEPC water
>
>Regards, 
>Wo
>
>> There are several products which are similar to "RNase Away".  I am
>> wondering which product works best for mRNA-related experiments.   Are
these
>> stuff a kind of EDTA or what?       jp
>> 
>> 
>> 
>
>
>-----
>Dr. Wolfgang Schechinger
>Institute of Biomedical Sciences
>Academia Sinica, Taipei, Taiwan R.o.C.
>
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>
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James Campanella,
Asst. Professor
Department of Biology and Molecular Biology
Montclair State University
1 Normal Avenue
Montclair, NJ 07043

Ph: 973-655-4097
Fax: 419-791-9834

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