Short PCR product

Rafael Maldonado rmaldonado at
Tue Jan 15 07:28:13 EST 2002

aes wrote:
> Hi.
> I am going to run PCR with 35 bp long primers, with an amplicon in between
> of 15 bp, so that the total lenght of the PCR product is 85 bp (for
> quantitation by real time pcr and sybr green1). My question is, what is the
> shortest posible amplicon? And is 15 bp to short?
> Thank you and happy new year

Amplicons of one base between the primers are possible. They are
obtained in single base mutation identification PCR.

Rafael Maldonado, Ph.D.
Division of Genetics
University of Alicante (Spain)

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