Short PCR product

[Rafael Maldonado]

aes wrote:
> 
> Hi.
> I am going to run PCR with 35 bp long primers, with an amplicon in between
> of 15 bp, so that the total lenght of the PCR product is 85 bp (for
> quantitation by real time pcr and sybr green1). My question is, what is the
> shortest posible amplicon? And is 15 bp to short?
> Thank you and happy new year

Amplicons of one base between the primers are possible. They are
obtained in single base mutation identification PCR.

-- 
Rafael Maldonado, Ph.D.
Division of Genetics
University of Alicante (Spain)