Short PCR product

Michael Witty mw132 at mole.bio.cam.ac.uk
Tue Jan 15 08:06:53 EST 2002


> > Hi.
> > I am going to run PCR with 35 bp long primers, with an amplicon in between
> > of 15 bp, so that the total lenght of the PCR product is 85 bp (for
> > quantitation by real time pcr and sybr green1). My question is, what is the
> > shortest posible amplicon? And is 15 bp to short?
> > Thank you and happy new year
>
> Amplicons of one base between the primers are possible. They are
> obtained in single base mutation identification PCR.

. . . there is also Ligase Chain Reaction where there are NO nucleotides
between the primers (or have I got this wrong?).  Mike.




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