Northern Blot troubles

Nick Jacobsen nickjacobsen at yahoo.co.uk
Wed Jan 16 10:00:18 EST 2002


Black spots can be caused by powder from rubber gloves. When preparing
a blot i try and avoid using gloves that have powder in them

hope this helps
Nick

> > From: "Theo Roemen" <theo.roemen at fys.unimaas.nl>
> > Organization: Universiteit Maastricht
> > Date: Fri, 11 Jan 2002 09:23:29 +0100
> > To: methods at hgmp.mrc.ac.uk
> > Subject: Northern Blot troubles
> > 
> > We separate RNA by Northern blotting and detection with labeled probes.
> > Since a few month we get many black spots on the membrane after using 32P-
> > labeled probes and after auto-radiography. Does anyone know what could be
> > the cause of this?
> > 
> 
> 
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