concentrating the protein

Amir amirsamani at hotmail.com
Thu Jan 17 00:40:23 EST 2002


I have a cell line which express a truncated or of IGF-1 receptor.  This
form of receptor is secreted out after expression.  To detect that I need
to  concentrate the condtioned medium.
1. What is the best way to concentrate proteins in medium?
2. Is there a way to protect the protein from proteases during the
concentrating?

Thanks


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