mw132 at mole.bio.cam.ac.uk
Thu Jan 17 07:27:41 EST 2002
> sweiss at icr.ac.uk wrote:
> >I am having major problems carrying out western blots. I ALWAYS end up with
> >a smear down the lane instead of a tight, compact band. I know that my
> >protein isn't degraded and that the antibodies are specific to the protein
> >and I have remade all my buffers. The only thing I can think of that might
> >be causing a problem is the acrylamide. I read that acrylamide can last up
> >to 9 months unopened and mine has been open for about 18 months, do you
> >think this could be affeecting my gels?
> Could be many things but don't blame it on acrylamide. Here we use
> some terrible junk given in large quantity for free about 8 years ago and
> it works fine for SDS-PAGE/westerns. Acrylamaide quality is only
> important for non-SDS applications.
. . . why is acrylamide quality only important for non-SDS applications?
More information about the Methods