D.K. dk at
Thu Jan 17 10:34:31 EST 2002

Michael Witty <mw132 at> wrote:
>> sweiss at wrote:
>> >
>> >I am having major problems carrying out western blots. I ALWAYS end up with
>> >a smear down the lane instead of a tight, compact band. I know that my
>> >protein isn't degraded and that the antibodies are specific to the protein
>> >and I have remade all my buffers. The only thing I can think of that might
>> >be causing a problem is the acrylamide. I read that acrylamide can last up
>> >to 9 months unopened and mine has been open for about 18 months, do you
>> >think this could be affeecting my gels?
>> >
>> Could be many things but don't blame it on acrylamide. Here we use
>> some terrible junk given in large quantity for free about 8 years ago and
>> it works fine for SDS-PAGE/westerns. Acrylamaide quality is only
>> important for non-SDS applications.
>.. . . why is acrylamide quality only important for non-SDS applications?

Because it is clearly not important for SDS-PAGE :-)


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