concentrating the protein

James Bassuk bassuk at u.washington.edu
Fri Jan 18 02:53:33 EST 2002


If all you need to do is run a gel, take your sample, add SDS to 0.1%, and
use the 0.5 ml MicroCon centrifugal concentrators.  OR, ppt with 10% TCA,
wash pellet with acetone/0.1%HCl, then acetone, dry briefly, then run gel.
Jim

On 17 Jan 2002, Amir wrote:

> I have a cell line which express a truncated or of IGF-1 receptor.  This
> form of receptor is secreted out after expression.  To detect that I need
> to  concentrate the condtioned medium.
> 1. What is the best way to concentrate proteins in medium?
> 2. Is there a way to protect the protein from proteases during the
> concentrating?
>
> Thanks
>
>
> <http://www.biowww.net/forum/read.php?f=1&i=5269&t=5269>
>
>




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