concentrating the protein
bassuk at u.washington.edu
Fri Jan 18 02:53:33 EST 2002
If all you need to do is run a gel, take your sample, add SDS to 0.1%, and
use the 0.5 ml MicroCon centrifugal concentrators. OR, ppt with 10% TCA,
wash pellet with acetone/0.1%HCl, then acetone, dry briefly, then run gel.
On 17 Jan 2002, Amir wrote:
> I have a cell line which express a truncated or of IGF-1 receptor. This
> form of receptor is secreted out after expression. To detect that I need
> to concentrate the condtioned medium.
> 1. What is the best way to concentrate proteins in medium?
> 2. Is there a way to protect the protein from proteases during the
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