bassuk at u.washington.edu
Fri Jan 18 02:51:21 EST 2002
This is due to junk not being soluble in sample buffer.
On 16 Jan 2002, Sophie wrote:
> I am having major problems carrying out western blots. I ALWAYS end up with
> a smear down the lane instead of a tight, compact band. I know that my
> protein isn't degraded and that the antibodies are specific to the protein
> and I have remade all my buffers. The only thing I can think of that might
> be causing a problem is the acrylamide. I read that acrylamide can last up
> to 9 months unopened and mine has been open for about 18 months, do you
> think this could be affeecting my gels?
> Any help and comments is much appreciated.
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