Smeared Western Samples

Sophie sweiss at icr.ac.uk
Fri Jan 18 05:04:26 EST 2002


I have found out the real reason for my rubbish, smeared western blot
results. The acrylamide had gone off. I don't think the acrylamide
polymerised properly so my protein was getting stuck to the gel instead of
migrating as a uniform band.

Sophie.


<http://www.biowww.net/forum/read.php?f=6&i=968&t=822>




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