Smeared Western Samples

Richard P. Grant rpg14 at yahoo.co.uk.invalid
Fri Jan 18 05:26:41 EST 2002


In article <20020118100426.16386.qmail at ww02.jatek.com>,
 Sophie <sweiss at icr.ac.uk> wrote:

> I have found out the real reason for my rubbish, smeared western blot
> results. The acrylamide had gone off. I don't think the acrylamide
> polymerised properly so my protein was getting stuck to the gel instead of
> migrating as a uniform band.

You got good results with new acrylamide, then?

When I was but a slip of a lad, my supervisor told me 'AMPS doesn't go 
off!' when we had problems getting gels to set.

Of course, it didn't take me long to realize the terrible, dreadful 
truth.

r

-- 
Richard P. Grant  0x5F9559B1                         MRC Lab of Mol Biol
rpg 'at' mrc-lmb.cam.ac.uk   http://www2.mrc-lmb.cam.ac.uk/personal/rpg/
               -- 'There's your answer, Fishbob!' --




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