Smeared Western Samples
Richard P. Grant
rpg14 at yahoo.co.uk.invalid
Fri Jan 18 05:26:41 EST 2002
In article <20020118100426.16386.qmail at ww02.jatek.com>,
Sophie <sweiss at icr.ac.uk> wrote:
> I have found out the real reason for my rubbish, smeared western blot
> results. The acrylamide had gone off. I don't think the acrylamide
> polymerised properly so my protein was getting stuck to the gel instead of
> migrating as a uniform band.
You got good results with new acrylamide, then?
When I was but a slip of a lad, my supervisor told me 'AMPS doesn't go
off!' when we had problems getting gels to set.
Of course, it didn't take me long to realize the terrible, dreadful
Richard P. Grant 0x5F9559B1 MRC Lab of Mol Biol
rpg 'at' mrc-lmb.cam.ac.uk http://www2.mrc-lmb.cam.ac.uk/personal/rpg/
-- 'There's your answer, Fishbob!' --
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