Northern Blot troubles

nieto at porthos.bio.ub.es nieto at porthos.bio.ub.es
Fri Jan 18 11:42:11 EST 2002


Hi fellows,

That happened to me once. I was using acrylamide-bis deionised with
mixed bed resin. The problem was that some resin had gone into the 
acrylamide solution after filtering.  I solved the problem 
filtering again the acrylamide stock.

Jose

> "Theo Roemen" <theo.roemen at fys.unimaas.nl> schreef in bericht
> news:a1m760$6pd$1 at rl0001.unimaas.nl...
> > We separate RNA by Northern blotting and detection with labeled probes.
> > Since a few month we get many black spots on the membrane after using 32P-
> > labeled probes and after auto-radiography. Does anyone know what could be
> > the cause of this?
> >
> >
> 
> 
> 
> 

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