leaky hot start Taq polymerase
roland.hubner at ua.ac.be
Mon Jan 21 08:43:23 EST 2002
> using platinum hot start polymerase I got all the
> same lots of primer dimers products, is it anybody else experience too?
> does anybody has a better source to suggest? what about the
> chemically modified ones, are they more expensive too?
==> "Rockstart" buffer is _EXCELLENT_ ( http://www.klentaq.com/ ); you
can drop the initial denaturation step completely... good for longish or
More information about the Methods