leaky hot start Taq polymerase

Roland Hubner roland.hubner at ua.ac.be
Mon Jan 21 08:43:23 EST 2002


> using platinum hot start polymerase I got all the
> same lots of primer dimers products, is it anybody else experience too?
> does anybody has a better source to suggest? what about the
> chemically modified ones, are they more expensive too?

Hello Vuvu!

==> "Rockstart" buffer is _EXCELLENT_  ( http://www.klentaq.com/ ); you 
can drop the initial denaturation step completely... good for longish or 
long-range amplifications...

Greetings,
 Roland




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