1/5X TE for long term storage of genomic DNA
Richard P. Grant
rpg14 at yahoo.co.uk.invalid
Tue Jan 22 07:40:50 EST 2002
In article <20020121221711.31797.qmail at ww02.jatek.com>,
Andre <arellano4 at llnl.gov> wrote:
> Ideally, I would like to resuspend and store the DNA and oligos long term in
> 1/5X TE (2mM Tris, 1mM EDTA)
>
>
> What do you think?
Is the DNA frozen or at 4C? The critical thing is the inhibition of
DNAses - and that's a function of the EDTA so you should be fine. If
you're freezing small aliquots then you might get away with pure,
unbuffered water.
--
Richard P. Grant 0x5F9559B1 MRC Lab of Mol Biol
rpg 'at' mrc-lmb.cam.ac.uk http://www2.mrc-lmb.cam.ac.uk/personal/rpg/
-- +44 1223 402261 - the Number of the Yeast --
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