1/5X TE for long term storage of genomic DNA

Richard P. Grant rpg14 at yahoo.co.uk.invalid
Tue Jan 22 07:40:50 EST 2002

In article <20020121221711.31797.qmail at ww02.jatek.com>,
 Andre <arellano4 at llnl.gov> wrote:

> Ideally, I would like to resuspend and store the DNA and oligos long term in
> 1/5X TE (2mM Tris, 1mM EDTA)
> What do you think?

Is the DNA frozen or at 4C?  The critical thing is the inhibition of 
DNAses - and that's a function of the EDTA so you should be fine.  If 
you're freezing small aliquots then you might get away with pure, 
unbuffered water.

Richard P. Grant  0x5F9559B1                         MRC Lab of Mol Biol
rpg 'at' mrc-lmb.cam.ac.uk   http://www2.mrc-lmb.cam.ac.uk/personal/rpg/
          -- +44 1223 402261 - the Number of the Yeast --

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