1/5X TE for long term storage of genomic DNA

Richard P. Grant rpg14 at yahoo.co.uk.invalid
Tue Jan 22 07:40:50 EST 2002


In article <20020121221711.31797.qmail at ww02.jatek.com>,
 Andre <arellano4 at llnl.gov> wrote:

> Ideally, I would like to resuspend and store the DNA and oligos long term in
> 1/5X TE (2mM Tris, 1mM EDTA)
> 
> 
> What do you think?

Is the DNA frozen or at 4C?  The critical thing is the inhibition of 
DNAses - and that's a function of the EDTA so you should be fine.  If 
you're freezing small aliquots then you might get away with pure, 
unbuffered water.

-- 
Richard P. Grant  0x5F9559B1                         MRC Lab of Mol Biol
rpg 'at' mrc-lmb.cam.ac.uk   http://www2.mrc-lmb.cam.ac.uk/personal/rpg/
          -- +44 1223 402261 - the Number of the Yeast --




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