1/5X TE for long term storage of genomic DNA

[Richard P. Grant]

In article 
<Pine.SGI.4.33.0201231500150.7148250-100000 at mole.bio.cam.ac.uk>,
 Michael Witty <mw132 at mole.bio.cam.ac.uk> wrote:

> My two cents: I tried purifying lambda DNA and storing in water at minus
> twenty degrees. It broke down and went all smearry.  Maybe my water was of
> poor quality, but since then I have always used the conventional T10E1
> buffer, pH8.

You probably had contaminating DNases that activated on thawing.

r

-- 
Richard P. Grant  0x5F9559B1                          MRC Lab of Mol Biol
rpg 'at' mrc-lmb.cam.ac.uk    http://www2.mrc-lmb.cam.ac.uk/personal/rpg/
                         'I have detailed files'