1/5X TE for long term storage of genomic DNA
Richard P. Grant
rpg14 at yahoo.co.uk.invalid
Wed Jan 23 10:13:52 EST 2002
<Pine.SGI.4.33.0201231500150.7148250-100000 at mole.bio.cam.ac.uk>,
Michael Witty <mw132 at mole.bio.cam.ac.uk> wrote:
> My two cents: I tried purifying lambda DNA and storing in water at minus
> twenty degrees. It broke down and went all smearry. Maybe my water was of
> poor quality, but since then I have always used the conventional T10E1
> buffer, pH8.
You probably had contaminating DNases that activated on thawing.
Richard P. Grant 0x5F9559B1 MRC Lab of Mol Biol
rpg 'at' mrc-lmb.cam.ac.uk http://www2.mrc-lmb.cam.ac.uk/personal/rpg/
'I have detailed files'
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