Trizol

BC bruno.removethis.cenni at removethis.insel.ch
Wed Jan 23 10:39:02 EST 2002


"Bertrand Collet" <b.collet at abdn.ac.uk> wrote in message
news:3C4EA5DB.A646D6D6 at abdn.ac.uk...
> Hi all,
>
> Is there anybody who uses Trizol to purify RNA, DNA and protein from the
> same tissue sample ? What about yield and purity of proteins and DNA ?
> (I have used it only for RNA extraction). And last question: Is it
> possible to use it on  blood ?
>
> Thanks for you help,
>
> Bertrand


Hi Bertrand,

I tried that once with cultured cells (roughly comparable to the buffy
coat...). No Problem with RNA and DNA, but the westerns looked awful (weak
signal for my protein of interest).

Bruno





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