mw132 at mole.bio.cam.ac.uk
Wed Jan 23 16:19:23 EST 2002
On 23 Jan 2002, Michael L. Sullivan wrote:
> >. . . anyone tried this? Or does it break down. Mike.
> I've used MES as a buffering agent in plant tissue culture media before,
> using an autoclaved stock and then re-autoclaving the media. From the pH
> adustment prior to the second autoclaving, the MES had certainly retained
> buffering capacity, although I couldn't tell you that there was no
> breakdown at all. It is certainly a fairly standard practice to make plant
> tissue culture media and autoclave it with the MES in it, but I suppose
> that doesn't mean there are no effects on the MES, just that that is how a
> lot of people do it!
> Hope this was somewhat helpful.
. . . Thanks Mike. I also phoned up the manufacturer: the real guy in the
Factory up north who makes it. He is a chemist and says it should be
"stable way above 120 degrees". I will bravely risk my 20x stock. But
tomorrow. Regards, Mike W.
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