1/5X TE for long term storage of genomic DNA
r.hartley at bio.gla.ac.uk
Thu Jan 24 06:29:09 EST 2002
"Richard P. Grant" <rpg14 at yahoo.co.uk.invalid> wrote in message
news:rpg14-CB2282.15135123012002 at pegasus.csx.cam.ac.uk...
> In article
> <Pine.SGI.4.33.0201231500150.7148250-100000 at mole.bio.cam.ac.uk>,
> Michael Witty <mw132 at mole.bio.cam.ac.uk> wrote:
> > My two cents: I tried purifying lambda DNA and storing in water at minus
> > twenty degrees. It broke down and went all smearry. Maybe my water was
> > poor quality, but since then I have always used the conventional T10E1
> > buffer, pH8.
> You probably had contaminating DNases that activated on thawing.
or the pH was low. remember the pH of your RO water is dependant on where
you live and is pretty acidic.
as a rough guess ,18ohm MiliQ is about pH6.
> Richard P. Grant 0x5F9559B1 MRC Lab of Mol Biol
> rpg 'at' mrc-lmb.cam.ac.uk http://www2.mrc-lmb.cam.ac.uk/personal/rpg/
> 'I have detailed files'
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