size for FISH

Nick Jacobsen nickjacobsen at yahoo.co.uk
Thu Jan 24 08:03:01 EST 2002


Hi Patrick,

this will be far too small for FISH. You really need a cosmid if
possible. Or in order to localise the microsatellite by PCR you could
use radiation hybrid mapping if you can get hold of a decent panel say
from Research Genetics.

hope this helps

cheers
Nick


Patrick <> wrote in message news:<20020123131525.24967.qmail at ww02.jatek.com>...
> Hello,
> 
> i would like to localize microsatellite (the total fragment size is about
> 300-350bp) at the chromosome level. Does somebody know if i can use FISH or
> is the fragment as i think too small?
> Thanks for any answer or advices,
> 
> Patrick
> 
> 
> <http://www.biowww.net/forum/read.php?f=1&i=5280&t=5280>




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