size for FISH
nickjacobsen at yahoo.co.uk
Thu Jan 24 08:03:01 EST 2002
this will be far too small for FISH. You really need a cosmid if
possible. Or in order to localise the microsatellite by PCR you could
use radiation hybrid mapping if you can get hold of a decent panel say
from Research Genetics.
hope this helps
Patrick <> wrote in message news:<20020123131525.24967.qmail at ww02.jatek.com>...
> i would like to localize microsatellite (the total fragment size is about
> 300-350bp) at the chromosome level. Does somebody know if i can use FISH or
> is the fragment as i think too small?
> Thanks for any answer or advices,
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