RT-PCR of highly coiled RNA

Irene ileeri at yahoo.com
Thu Jan 24 17:03:20 EST 2002

I have been trying to amplify by RT-PCR a region with very high secondary
structure. In order to reduce this I have tried to rise the temperature, add
DMSO, change the KCl concentration, and add glicerol and even formamide.
Nothings seems to work! Any suggestion?


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