I have been trying to amplify by RT-PCR a region with very high secondary structure. In order to reduce this I have tried to rise the temperature, add DMSO, change the KCl concentration, and add glicerol and even formamide. Nothings seems to work! Any suggestion? <http://www.biowww.net/forum/read.php?f=1&i=5281&t=5281>