SDS-PAGE of Serum Sample

Florence wythum at yahoo.com
Fri Jan 25 02:29:45 EST 2002


Dear All,

I'm running SDS-PAGE with serum samples, but I'm having a serious problem
that there's always a clump of unknown protein aggregates between 98-50 kD,
and the lanes become twisted.  I've tried to reduce total protein mass to 50
ug, or purify protein from serum sample using TCA precipitation, but still
can't solve this problem.

Can someone provide any suggestion?


Thanks,
Florence


<http://www.biowww.net/forum/read.php?f=6&i=971&t=971>




More information about the Methods mailing list