cloning PCR products

Purvi Bhatt purvi_bhatt75 at
Fri Jan 25 07:30:01 EST 2002

Hi all,
i too am facing a lot of problems with my 2kb insert which i am trying to
clone into expression vector. I fail to understand why i get a  lot of
colonies on vector self ligated plate while the test plate shows less
colonies. also i am not getting even a single recombinant???? pl help as to
where  can i be going wrong, is my vector not completely digested or what????
troubleshooting needed. thanx.


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