RT-PCR of highly coiled RNA

Michael Witty mw132 at mole.bio.cam.ac.uk
Fri Jan 25 09:32:02 EST 2002


On 25 Jan 2002, Roswitha wrote:

> Have you tried heating your RNA to 65=B0C for 5 min before adding it to y=
our
> reaction mixture (containing DMSO. for example) ?

 . . . I often see people recommend DMSO for PCRs: what suppliers do you
use.  How much in a reaction and how do you store it?  Regards, Mike.




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