Help-EM7 promoter

RV rvisvanathan at hotmail.com
Sat Jan 26 16:01:26 EST 2002


Hi ,

I am planning to invitro transcribe/translate a piece DNA cloned into
pCDNA4His MAX (invitrogen)
using the T7 promoter present in the vector. Unfortunately there is a zeocin
gene which is driven
by an synthetic T7 promoter. Will that gene also be transcribed by T7
polymerase? In short my question is Will T7 polymerase recognize this EM-7
promoter?

Thanks for your help/suggestions,
Vishy
visu at u.washington.edu





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