RT-PCR of highly coiled RNA

Roland Hübner roland.hubner at ua.ac.be
Mon Jan 28 05:46:36 EST 2002


> I have been trying to amplify by RT-PCR a region with very high secondary
> structure. In order to reduce this I have tried to rise the temperature, 
> add
> DMSO, change the KCl concentration, and add glicerol and even formamide.
> Nothings seems to work! Any suggestion?

Hi,

 what was the "highest" temperature you tried so far ?

R.




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