single strand DNA PCR

Sergio seraln at _NO_SPAM_netscape.net
Wed Jan 30 10:46:39 EST 2002


I would say that any polymerase should work, since ssDNA is what they work on 
when doing normal PCR, after denaturation step. When we perform RT-PCR we use a 
normal Taq polymerase for the amplification (after the RT step), and all what 
the enzyme finds is ssDNA.
I've never tried with a ssDNA sample, though.
Sergio


kelvin wrote:

> Does anyone have the experience of single strand DNA PCR? I am trying to
> amplify single strand DNA from 100-1000ng starting thing.What kind of enzyme
> is most efficient?
> Thanks a lot.
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> <http://www.biowww.net/forum/read.php?f=1&i=5287&t=5287>
> 




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