Which cross-linking reagent?

Kyle Legate legatek at mcmail.cis.mcmaster.ca
Wed Jan 30 13:18:16 EST 2002


On Wed, 30 Jan 2002, Ricky Boernke wrote:

> we would like to cross-link a bait protein expressed in
> Arabidopsis to potential binding partners and subsequently
> purify the complex using our protein as a tag.
> However, we are quiete unsure which cross-linking reagent
> we should use for this purpose. The Pierce catalogue offers
> quiete a few different types and I would suppose they are all
> suited for different purposes. Is there anything like a
> guide to protein cross-linking?
>
I found the Pierce catalogue to be the best guide for cross-linking
principles. Molecular Probes has some good info as well.

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legatek at mcmaster.ca		Kyle Legate            legatek at hotmail.com

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