noncomplementary primers amd pfu polymerase

PAtrick Lynch patrick.lynch at
Wed Jan 30 14:54:28 EST 2002


I'm trying to amplify a 0.5 kb fragment of DNA using a set of primers, one
of which has a 5'-27 base tail that isn't complementary to the initial
template, with pfu polymerase (cloned - Stratagene). Unfortunately, the PCR
never works. This problem of 5'-noncomplementary primer ends and
amplification with pfu has been reported before in this NG. I wonder if
anyone has ever succeeded in doing this sort of amplification with pfu?




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